Part:BBa_I739008

Double regulated intermediate for cI +LVA (I739103.B0034.C0051)

Part Structure

The Biobrick encodes LVA-tagged cI (BBa_C0051) under control of the double promoter BBa_I739103 followed by the ribosome binding site BBa_B0034. Please notice that there is no terminator in this construct. If you plan to use this construct for double regulated cI production, you should combine it with an appropriate terminator (e.g. BBa_B0015).

Mode of Action

cI production can be repressed by lacI and/or P22 cII. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of P22 cII.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is also termed Part 8. The synthesized cI interacts with the double promoters BBa_I739105, BBa_I739102 and BBa_I739107 which are parts of composites BBa_I739007, BBa_I739015 and BBa_I739011 respectively. Together with Part 9, this Biobrick forms the composite part BBa_I739016.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]