Part:BBa_I739009

Promoterless expression cassette for ECFP +LVA (B0034.E0022.B0015)

Part Structure

The Biobrick encodes LVA-tagged ECFP (BBa_E0022) downstream of the ribosome binding site BBa_B0034. The transcription of ECFP is terminated by the double terminator BBa_B0015. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct. This synthically synthesized part is identical to part BBa_E0422.

Mode of Action

Enhanced cyan fluorescent proteins (ECFP) are fluorochromes, commonly used as reporter proteins.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is also termed Part 9. Together with Part 8, this Biobrick forms the composite part BBa_I739016.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]