User contributions
(newest | oldest) View (newer 100 | older 100) (20 | 50 | 100 | 250 | 500)
- 13:16, 28 October 2017 (diff | hist) . . (+410) . . Part:BBa I0500:Experience (→Advice)
- 13:13, 28 October 2017 (diff | hist) . . (+337) . . Part:BBa I0500:Experience (→Method)
- 12:59, 28 October 2017 (diff | hist) . . (+1) . . Part:BBa I0500:Experience (→Contribution iGEM TU-Eindhoven 2017 http://2017.igem.org/Team:TU-Eindhoven])
- 12:58, 28 October 2017 (diff | hist) . . (-355) . . Part:BBa I0500:Experience (→Advice)
- 12:56, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector mCherry and GFP best results.png (current)
- 12:56, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector GFP best results.png (current)
- 12:54, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector GFP 90min.png (current)
- 12:54, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector GFP 60min.png (current)
- 12:53, 28 October 2017 (diff | hist) . . (+1,294) . . Part:BBa I0500:Experience
- 12:42, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector mCherry best results.png (current)
- 12:41, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector mCherry 90min.png (current)
- 12:40, 28 October 2017 (diff | hist) . . (0) . . N File:T-TU-Eindhoven--pBAD vector mCherry 60min.png (current)
- 12:39, 28 October 2017 (diff | hist) . . (+240) . . Part:BBa I0500:Experience (→mCherry)
- 12:37, 28 October 2017 (diff | hist) . . (+1,123) . . Part:BBa I0500:Experience
- 12:29, 28 October 2017 (diff | hist) . . (+46) . . N File:T--TU-Eindhoven--pBAD vector GFP best results.png (Results of expression by addition of arabinose) (current)
- 12:17, 28 October 2017 (diff | hist) . . (+31) . . Part:BBa I0500
- 12:16, 28 October 2017 (diff | hist) . . (+110) . . Part:BBa I0500
- 01:06, 19 September 2015 (diff | hist) . . (+286) . . Part:BBa J23101:Experience (TU Eindhoven 2015 experience)
- 00:51, 19 September 2015 (diff | hist) . . (+63) . . Part:BBa K1761000:Design (current)
- 00:46, 19 September 2015 (diff | hist) . . (+273) . . Part:BBa K1761000:Design
- 00:33, 19 September 2015 (diff | hist) . . (+59) . . Part:BBa K1761006 (→Characterization)
- 00:32, 19 September 2015 (diff | hist) . . (+59) . . Part:BBa K1761005 (→Characterization)
- 00:25, 19 September 2015 (diff | hist) . . (+58) . . Part:BBa K1761002 (→Characterization)
- 00:25, 19 September 2015 (diff | hist) . . (+58) . . Part:BBa K1761001 (→Characterization)
- 00:24, 19 September 2015 (diff | hist) . . (+59) . . Part:BBa K1761000 (→Characterization)
- 00:19, 19 September 2015 (diff | hist) . . (0) . . Part:BBa K1761003 (→Usage and Biology)
- 00:18, 19 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761001 (→Usage and Biology)
- 00:12, 19 September 2015 (diff | hist) . . (-35) . . Part:BBa K1761003 (→Characterization part of a construct)
- 00:05, 19 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761003 (→Measurement with the Tecan Safire2 platereader)
- 23:34, 18 September 2015 (diff | hist) . . (+177) . . Part:BBa K1761003:Experience
- 23:28, 18 September 2015 (diff | hist) . . (+4) . . Part:BBa K1761003 (figures)
- 23:26, 18 September 2015 (diff | hist) . . (+392) . . N File:TU Eindhoven mNeonGreen Safire2.png (The data for the three biological replicates shows high intensity peaks at approximately 517 nm. Three technical replicates for each of the biological replicates were measured. The average of these three technical replicates was used for the shown spectra) (current)
- 23:26, 18 September 2015 (diff | hist) . . (+69) . . N File:TU Eindhoven mNeonGreen Construct3.png (Spectrum of mNeonGreen and constitutive promotor J23101, Construct 3.) (current)
- 23:25, 18 September 2015 (diff | hist) . . (+69) . . N File:TU Eindhoven mNeonGreen Construct2.png (Spectrum of mNeonGreen and constitutive promotor J23101, Construct 2.) (current)
- 23:25, 18 September 2015 (diff | hist) . . (+69) . . N File:TU Eindhoven mNeonGreen Construct1.png (Spectrum of mNeonGreen and constitutive promotor J23101, Construct 1.) (current)
- 23:23, 18 September 2015 (diff | hist) . . (+397) . . N File:TU Eindhoven mNeonGreen EmissionScan.png (An in vivo emissions scan was conducted for mNeonGreen in the BL21(DE3) cells. The cells containing mNeonGreen show a peak at 506 nm. The first negative control, BL21(DE3) cells with the empty pSB1C3 plasmid do not show such a peak. The second negative co) (current)
- 23:23, 18 September 2015 (diff | hist) . . (+379) . . N File:TU Eindhoven mNeonGreen ExcitationScan.png (The pSB1C3 vector containing mNeonGreen vector was transformed into BL21(DE3) cells. These cells were measured at an OD of 2.0. Three biological triplicates were obtained which all showed peaks at approximately 517 nm. The first negative control, cells wi) (current)
- 23:22, 18 September 2015 (diff | hist) . . (+3,876) . . Part:BBa K1761003 (characterization)
- 22:46, 18 September 2015 (diff | hist) . . (+221) . . Part:BBa K1761006:Experience
- 22:45, 18 September 2015 (diff | hist) . . (+200) . . Part:BBa K1761005:Experience
- 22:24, 18 September 2015 (diff | hist) . . (+399) . . Part:BBa K1761001 (NanoBit)
- 22:19, 18 September 2015 (diff | hist) . . (+239) . . Part:BBa K1761000 (figure)
- 22:05, 18 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761006
- 22:04, 18 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761005
- 21:59, 18 September 2015 (diff | hist) . . (+147) . . Part:BBa K1761002:Experience (promega) (current)
- 21:54, 18 September 2015 (diff | hist) . . (+311) . . Part:BBa K1761006 (References)
- 21:53, 18 September 2015 (diff | hist) . . (+311) . . Part:BBa K1761005 (References)
- 21:43, 18 September 2015 (diff | hist) . . (+9) . . Part:BBa K1761006 (Bioluminiscence NanoBit)
- 21:40, 18 September 2015 (diff | hist) . . (+2) . . Part:BBa K1761005:Experience
- 21:38, 18 September 2015 (diff | hist) . . (+41) . . Part:BBa K1761005:Experience
- 21:36, 18 September 2015 (diff | hist) . . (+433) . . Part:BBa K1761006:Design (current)
- 21:35, 18 September 2015 (diff | hist) . . (+241) . . Part:BBa K1761005:Design (current)
- 21:16, 18 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761006
- 21:16, 18 September 2015 (diff | hist) . . (+2) . . Part:BBa K1761005
- 20:43, 18 September 2015 (diff | hist) . . (+191) . . Part:BBa K1761005:Design (promega)
- 20:33, 18 September 2015 (diff | hist) . . (-1) . . Part:BBa K1761005 (volgorde)
- 20:29, 18 September 2015 (diff | hist) . . (+652) . . Part:BBa K1761003:Experience (application)
- 20:09, 18 September 2015 (diff | hist) . . (+1,236) . . Part:BBa K1761003 (Bioluminiscence)
- 20:03, 18 September 2015 (diff | hist) . . (+6) . . Part:BBa K1761003 (figure)
- 20:02, 18 September 2015 (diff | hist) . . (+230) . . N File:TU Eindhoven mNeonGreen SnapGene.png (Snapgene plasmid overview of the BioBrick part BBa_K1761003. It shows the pSB1C3 vector with the prefix (containing the restriction sites EcoRI and XbaI), mNeonGreen and the suffix (containting the restiction sites SpeI and PstI).) (current)
- 19:35, 18 September 2015 (diff | hist) . . (+983) . . Part:BBa K1761002 (Bioluminiscence)
- 19:31, 18 September 2015 (diff | hist) . . (+682) . . N File:TU Eindhoven results bioluminiscence neongreen and NanoLuc.png (OmpX-NanoLuc's & OmpX-mNeonGreen's presence in the cells were tested by adding Nano-Glo substrate to the cells and measuring bioluminescence with the spectrophotometer. The figure below shows that OmpX-NanoLuc shows a peak characteristic for NanoLuc, indi) (current)
- 19:01, 18 September 2015 (diff | hist) . . (+2) . . Part:BBa K1761000:Design (unnatural amino acid)
- 11:45, 18 September 2015 (diff | hist) . . (+203) . . Part:BBa K1761006:Experience (Experience TU Eindhoven 2015)
- 11:45, 18 September 2015 (diff | hist) . . (0) . . Part:BBa K1761005:Experience (Experience TU Eindhoven 2015)
- 11:42, 18 September 2015 (diff | hist) . . (+32) . . Part:BBa K1761005:Experience (→Applications of BBa_K1761005)
- 11:38, 18 September 2015 (diff | hist) . . (+170) . . Part:BBa K1761005:Experience (aplication)
- 11:31, 18 September 2015 (diff | hist) . . (+337) . . Part:BBa K1761003:Experience (BRET couple)
- 11:21, 18 September 2015 (diff | hist) . . (-8) . . Part:BBa K1761000:Design (non-natural into unnatural)
- 09:37, 18 September 2015 (diff | hist) . . (-8) . . Part:BBa K1761006 (non-natural into unnatural)
- 09:36, 18 September 2015 (diff | hist) . . (-8) . . Part:BBa K1761005 (non-natural into unnatural)
- 09:36, 18 September 2015 (diff | hist) . . (-2) . . Part:BBa K1761004 (non-natural into unnatural) (current)
- 09:35, 18 September 2015 (diff | hist) . . (-2) . . Part:BBa K1761003 (non-natural into unnatural)
- 09:35, 18 September 2015 (diff | hist) . . (-10) . . Part:BBa K1761002 (non-natural into unnatural)
- 09:34, 18 September 2015 (diff | hist) . . (-10) . . Part:BBa K1761001 (non-natural into unnatural)
- 09:34, 18 September 2015 (diff | hist) . . (-14) . . Part:BBa K1761000 (non-natural into unnatural)
- 06:55, 18 September 2015 (diff | hist) . . (0) . . Part:BBa K1761005
- 06:55, 18 September 2015 (diff | hist) . . (+52) . . N File:TU Eindhoven Results NanoBit bioluminescence.png (Bioluminescence of results of the NanoBit construct.) (current)
- 06:52, 18 September 2015 (diff | hist) . . (+8) . . Part:BBa K1761005 (figures)
- 06:50, 18 September 2015 (diff | hist) . . (+98) . . N File:TU Eindhoven FACS OmpX NanoBit.png (FACS results of the OmpX - LargeBit construct and the OmpX - SmallBit construct, together NanoBit.) (current)
- 06:47, 18 September 2015 (diff | hist) . . (+45) . . N File:TU Eindhoven FACS OmpX SmBit.png (FACS results of the OmpX - SmallBit construct) (current)
- 06:46, 18 September 2015 (diff | hist) . . (+46) . . N File:TU Eindhoven FACS OmpX LgBit.png (FACS results of the OmpX - LargeBit construct.) (current)
- 06:45, 18 September 2015 (diff | hist) . . (+45) . . N File:TU Eindhoven OmpX LgBiT and OmpX SmBiT.png (Schematical overview of the NanoBit construct) (current)
- 06:44, 18 September 2015 (diff | hist) . . (+62) . . N File:TU Eindhoven Construct OmpX SmBiT.png (Schematical overview of the OmpX (1) - SmallBit (2) construct.) (current)
- 06:44, 18 September 2015 (diff | hist) . . (+62) . . N File:TU Eindhoven Construct OmpX LgBiT.png (Schematical overview of the OmpX (1) - LargeBit (2) construct.) (current)
- 18:56, 17 September 2015 (diff | hist) . . (-2) . . Part:BBa K1761005
- 18:56, 17 September 2015 (diff | hist) . . (+4,298) . . Part:BBa K1761006 (Documentation)
- 18:52, 17 September 2015 (diff | hist) . . (+408) . . Part:BBa K1761005 (figures)
- 18:33, 17 September 2015 (diff | hist) . . (+3,893) . . Part:BBa K1761005 (general)
- 18:58, 16 September 2015 (diff | hist) . . (0) . . File:TU Eindhoven Constructs OmpX NG and OmpX NL.png (uploaded a new version of "File:TU Eindhoven Constructs OmpX NG and OmpX NL.png": cropped) (current)
- 18:57, 16 September 2015 (diff | hist) . . (0) . . File:TU Eindhoven Construct OmpX NanoLuc.png (uploaded a new version of "File:TU Eindhoven Construct OmpX NanoLuc.png": cropped) (current)
- 18:56, 16 September 2015 (diff | hist) . . (0) . . File:TU Eindhoven Construct OmpX mNeonGreen.png (uploaded a new version of "File:TU Eindhoven Construct OmpX mNeonGreen.png": cropped) (current)
- 18:40, 16 September 2015 (diff | hist) . . (+3,133) . . Part:BBa K1761003:Design (the signaling component) (current)
- 18:04, 16 September 2015 (diff | hist) . . (+2,239) . . Part:BBa K1761002:Experience
- 18:02, 16 September 2015 (diff | hist) . . (+2,239) . . Part:BBa K1761001:Experience (Experience TU Eindhoven 2015) (current)
- 17:56, 16 September 2015 (diff | hist) . . (+404) . . N File:TU Eindhoven DNA strand displacement System.png (The membrane proteins can be brought in close proximity by clicking oligonucleotides on the loops and adding a long strand complementary to both oligonucleotides. It is expected that this results in a measurable signal. The longer strand is functionalized) (current)
- 17:55, 16 September 2015 (diff | hist) . . (+291) . . N File:TU Eindhoven DNA strand displacement.png (Schematic overview of DNA strand displacement. DNA Strand displacement is initiated when the input binds to the toehold region of a partially hybridized DNA strand. After the initiation, branch migration takes place and the input fully hybridizes with its) (current)
- 17:53, 16 September 2015 (diff | hist) . . (+2,332) . . Part:BBa K1761000:Experience (TU Eindhoven 2015 experience) (current)
- 17:47, 16 September 2015 (diff | hist) . . (+356) . . N File:TU Eindhoven Oligos verification.png (To verify whether the click reaction has occured with DBCO-modified DNA, we incubate the cells with DBCO-functionalized complementary strands. If the DBCO-modified DNA clicks, the fluorescently labeled DBCO-functionalized complementary strands will anneal) (current)
- 18:21, 15 September 2015 (diff | hist) . . (+3,660) . . Part:BBa K1761002:Design (signaling components) (current)
(newest | oldest) View (newer 100 | older 100) (20 | 50 | 100 | 250 | 500)