User contributions
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- 13:38, 9 October 2018 (diff | hist) . . (+127) . . Part:BBa K2586021
- 13:30, 9 October 2018 (diff | hist) . . (+27,892) . . Part:BBa K2586021
- 13:29, 9 October 2018 (diff | hist) . . (+7,043) . . Part:BBa K2586002
- 13:26, 9 October 2018 (diff | hist) . . (+7,064) . . Part:BBa K2586001
- 13:20, 9 October 2018 (diff | hist) . . (+293) . . N File:T--Goettingen--competitionassay 180911 hi.png (The fluorescence is normed to the optical density, which equals the cell number. A: With increasing glyphosate concentrations (0–2 mM glyphosate) decreases the GFP fluorescence. B: With increasing glyphosate concentrations (0–2 mM glyphosate) is th...) (current)
- 12:44, 9 October 2018 (diff | hist) . . (+763) . . N File:T--Goettingen--competitionassay microscopemitauswertungneu h.png (Green cells represent the wildtype strain (trpC2 amyE::(morange-lacZ aphA3)) and orange cells the double transporter mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(morange-lacZ aphA3)). The ratio indicates the relative amount of orange cells in compariso...) (current)
- 12:17, 9 October 2018 (diff | hist) . . (+462) . . N File:T--Goettingen--competitionassay growthderorangenen.png (Growth of strains: 168: wildtype. iGEM24: mOrange-tagged wildtype (trpC2 amyE::(mOrange-lacZ aphA3)). iGEM28: mOrange-tagged ΔgltT mutant (trpC2 ΔgltT::spc amyE::(mOrange-lacZ aphA3)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc Δ...) (current)
- 10:48, 9 October 2018 (diff | hist) . . (+454) . . N File:T--Goettingen--competitionassay overview als jpg mithintergrund.png.jpg (The basic principle of the competition assay for glyphosate detection. In the absence of glyphosate (no selective pressure), both strains grow with the same rate. When glyphosate is present, the herbicide-resistant strain (expressing an orange fluoroph...) (current)
- 10:39, 9 October 2018 (diff | hist) . . (+8,051) . . Part:BBa K2586019 (current)
- 10:28, 9 October 2018 (diff | hist) . . (+10,060) . . Part:BBa K2586019
- 10:15, 9 October 2018 (diff | hist) . . (+422) . . N File:T--Goettingen--ratearoagat.png (The relationship between the growth rate (µ) and the glyphosate (GS) concentration for the B. subtilis wild type (WT) strain SP1, the ΔgltT mutant strain BP233, the gat B. subtilis strain, the aroA* B. subtilis strain, the B. subtilis strain containi...) (current)
- 10:14, 9 October 2018 (diff | hist) . . (+743) . . N File:T--Goettingen--growtharoa-gat.png (A. Growth of the B. subtilis wild type strain in presence of 0 - 3.5 mM Glyphosate. B. Growth of the ∆gltT B. subtilis strain with increasing glyphosate concentration (0-30 mM). C. Growth of the gat B. subtilis strain with increasing glyphosate conce...) (current)
- 09:57, 9 October 2018 (diff | hist) . . (+315) . . N File:T--goettingen--roundup aroA gat sp1.jpg (A. Growth of the wild type and ΔgltT mutant and of the isogenic strains overexpressing the gat R11 and aroA* genes on CS-Glc minimal medium in the presence of different amounts of glyphosate. B. Growth of the strains described in A on CS-Glc minimal m...) (current)
- 09:27, 9 October 2018 (diff | hist) . . (+1,578) . . N File:T--goettingen--gat engineering.jpg (A. Sequence alignment showing the similarities of the wild type (wt) gat alleles from Bacillus licheniformis and Bacillus subtilis as well as the gat alleles R7 and R11 from B. licheniformis that were obtained by 11 rounds of gene shuffling. The red ar...) (current)
- 08:47, 9 October 2018 (diff | hist) . . (+230) . . N File:T--goettingen--structure GAT.png (The Molecular Basis of Glyphosate Resistance by an Optimized Microbial Acetyltransferase. Siehl, D.L., Castle, L.A., Gorton, R., Keenan, R.J. (2007) J.Biol.Chem. 282: 11446 (https://www.rcsb.org/structure/2jdd, 09.10.2018 10:46)) (current)
- 16:45, 24 September 2018 (diff | hist) . . (-1) . . Part:BBa K2586003 (→Purification and ICTS with aroA) (current)
- 16:42, 24 September 2018 (diff | hist) . . (+20,490) . . Part:BBa K2586011 (current)
- 14:07, 24 September 2018 (diff | hist) . . (-126) . . Part:BBa K2586003
- 14:04, 24 September 2018 (diff | hist) . . (+6,050) . . Part:BBa K2586003
- 12:18, 24 September 2018 (diff | hist) . . (+12) . . Part:BBa K2586002
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