File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 13:50, 9 October 2018 | T--Goettingen--gfp-xgal.png (file) |  |
44 KB | Cell mixture (1:1) of strains BP193 and BP235 were incubated with increasing glyphosate concentrations (0–5 mM). Strain BP193 harbors the lacZ gene behind an artificial promoter, but does not tolerate glyphosate in contrast to strain BP235. | 1 |
| 13:46, 9 October 2018 | T--Goettingen--competitionassay blue.png.jpg (file) |  |
84 KB | Cell mixture (1:1) of strains BP193 and BP235 were incubated with increasing glyphosate concentrations (0–5 mM). Strain BP193 harbors the lacZ gene behind an artificial promoter, but does not tolerate glyphosate in contrast to strain BP235. The inten... | 1 |
| 13:20, 9 October 2018 | T--Goettingen--competitionassay 180911 hi.png (file) |  |
89 KB | The fluorescence is normed to the optical density, which equals the cell number. A: With increasing glyphosate concentrations (0–2 mM glyphosate) decreases the GFP fluorescence. B: With increasing glyphosate concentrations (0–2 mM glyphosate) is th... | 1 |
| 12:44, 9 October 2018 | T--Goettingen--competitionassay microscopemitauswertungneu h.png (file) |  |
144 KB | Green cells represent the wildtype strain (trpC2 amyE::(morange-lacZ aphA3)) and orange cells the double transporter mutant (trpC2 ΔgltT::spc ΔgltP::cat amyE::(morange-lacZ aphA3)). The ratio indicates the relative amount of orange cells in compariso... | 1 |
| 12:17, 9 October 2018 | T--Goettingen--competitionassay growthderorangenen.png (file) |  |
10 KB | Growth of strains: 168: wildtype. iGEM24: mOrange-tagged wildtype (trpC2 amyE::(mOrange-lacZ aphA3)). iGEM28: mOrange-tagged ΔgltT mutant (trpC2 ΔgltT::spc amyE::(mOrange-lacZ aphA3)). iGEM36: mOrange-tagged ΔgltT ΔgltP mutant (trpC2 ΔgltT::spc Δ... | 1 |
| 10:48, 9 October 2018 | T--Goettingen--competitionassay overview als jpg mithintergrund.png.jpg (file) |  |
67 KB | The basic principle of the competition assay for glyphosate detection. In the absence of glyphosate (no selective pressure), both strains grow with the same rate. When glyphosate is present, the herbicide-resistant strain (expressing an orange fluoroph... | 1 |
| 10:15, 9 October 2018 | T--Goettingen--ratearoagat.png (file) |  |
101 KB | The relationship between the growth rate (µ) and the glyphosate (GS) concentration for the B. subtilis wild type (WT) strain SP1, the ΔgltT mutant strain BP233, the gat B. subtilis strain, the aroA* B. subtilis strain, the B. subtilis strain containi... | 1 |
| 10:14, 9 October 2018 | T--Goettingen--growtharoa-gat.png (file) |  |
209 KB | A. Growth of the B. subtilis wild type strain in presence of 0 - 3.5 mM Glyphosate. B. Growth of the ∆gltT B. subtilis strain with increasing glyphosate concentration (0-30 mM). C. Growth of the gat B. subtilis strain with increasing glyphosate conce... | 1 |
| 09:57, 9 October 2018 | T--goettingen--roundup aroA gat sp1.jpg (file) |  |
332 KB | A. Growth of the wild type and ΔgltT mutant and of the isogenic strains overexpressing the gat R11 and aroA* genes on CS-Glc minimal medium in the presence of different amounts of glyphosate. B. Growth of the strains described in A on CS-Glc minimal m... | 1 |
| 09:27, 9 October 2018 | T--goettingen--gat engineering.jpg (file) |  |
722 KB | A. Sequence alignment showing the similarities of the wild type (wt) gat alleles from Bacillus licheniformis and Bacillus subtilis as well as the gat alleles R7 and R11 from B. licheniformis that were obtained by 11 rounds of gene shuffling. The red ar... | 1 |
| 08:47, 9 October 2018 | T--goettingen--structure GAT.png (file) |  |
41 KB | The Molecular Basis of Glyphosate Resistance by an Optimized Microbial Acetyltransferase. Siehl, D.L., Castle, L.A., Gorton, R., Keenan, R.J. (2007) J.Biol.Chem. 282: 11446 (https://www.rcsb.org/structure/2jdd, 09.10.2018 10:46) | 1 |
| 12:01, 24 September 2018 | T--goettingen--iC50.jpg (file) |  |
280 KB | The relationship between the growth rate (µ) and the glyphosate (GS) concentration for the B. subtilis wild type (WT) strain 168, the ΔgltT mutant strain BP233 and the the ΔgltT ΔgltP mutant strain BP235 in CS-Glc minimal medium supplemented with i... | 1 |
| 11:35, 24 September 2018 | T--goettingen--gltT mutations clean.jpg (file) |  |
893 KB | Inactivation of the glutamate transporters confers high-level resistance to glyphosate. (A) Growth of the B. subtilis wild type (WT) strain 168 in CS-Glc minimal medium supplemented with increasing amounts of glyphosate (GS). (B) Growth of the ΔgltT m... | 1 |
| 10:41, 24 September 2018 | T--goettingen--BBa K2586002 structureGltP.png (file) |  |
210 KB | Crystal structure of a substrate-free aspartate transporter. Jensen, S., Guskov, A., Rempel, S., Hanelt, I., Slotboom, D.J. (2013) Nat.Struct.Mol.Biol. 20: 1224-1226 (http://www.rcsb.org/structure/4KY0, 24.09.18 12:41) | 1 |
| 16:57, 23 September 2018 | T--goettingen--aroE aroA Strep ITC Marburg.jpg (file) |  |
697 KB | (A) Evaluation of the purification of the N-terminally Strep-tagged EPSP synthases from B. subtilis and E. coli Strep-AroE and Strep-AroA, respectively, by 12% SDS PAGE. The proteins were stained with Coomassie Brilliant Blue. M, unstained protein mole... | 1 |
| 16:52, 23 September 2018 | T--goettingen--aroE essentiality.jpg (file) |  |
358 KB | The EPSP synthase is essential in B. subtilis. Transformation experiment to evaluate the essentiality of the EPSP synthase in B. subtilis. The bacteria were propagated on SP plates supplemented with spectinomycin and incubated for 24 h at 37°C. | 1 |
| 16:50, 23 September 2018 | T--goettingen--BBa K2586007 structureAroA.jpg (file) |  |
62 KB | Structural Basis of Glyphosate Resistance Resulting from the Double Mutation Thr97 -> Ile and Pro101 -> Ser in 5-Enolpyruvylshikimate-3-phosphate Synthase from Escherichia coli. Funke, T., Yang, Y., Han, H., Healy-Fried, M., Olesen, S., Becker, A., Sch... | 1 |
| 16:34, 23 September 2018 | T--goettingen--BBa K2586007 structureGltT.png (file) |  |
205 KB | Binding thermodynamics of a glutamate transporter homolog. Reyes, N., Oh, S., Boudker, O. (2013) Nat.Struct.Mol.Biol. 20: 634-640 (http://www.rcsb.org/structure/4IZM, 23.09.2018 18:33) | 1 |
| 16:25, 23 September 2018 | T--goettingen--BBa K2586007 structureAroE.jpg (file) |  |
74 KB | Crystal structure of putative 5-enolpyruvoylshikimate-3-phosphate synthase from Bacillus halodurans C-125 Malashkevich, V.N., Toro, R., Seidel, R., Ramagopal, U., Zencheck, W., Almo, S.C. (http://www.rcsb.org/structure/3RMT, 23.09.2018 18:23) | 1 |
| 15:28, 23 September 2018 | T--Goettingen--BBa K2586007 aroAstructure.png (file) |  |
76 KB | Crystal structure of Phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase) (TM0343) from Thermotoga Maritima at 1.92 A resolution Joint Center for Structural Genomics (JCSG) (http://www.rcsb.org/structure/1VR6, 23.09.2018-17:28) | 1 |
| 12:02, 22 September 2018 | T--goettingen--GS targets mechanisms.jpg (file) |  |
348 KB | Mechanisms conferring resistance to glyphosate. GltT and GltP, B. subtilis glutamate transporters; YhhS and MFS40, major facilitator secondary transporters from E. coli and A. oryzae; GAT, B. licheniformis glyphosate N-acetyltransferase; GlpA, P. pseud... | 1 |
| 11:41, 22 September 2018 | T--goettingen--B. subtilis Glufosinate.jpg (file) |  |
328 KB | Glufosinate is also taken up by B. subtilis via GltT. (A) Comparison of glutamate and glufosinate. (B) Disk diffusion assay to test whether glufosinate is transported via GltT and/or GltP. The strains 168 (wild type), BP233 (ΔgltT), BP234 (ΔgltP, BP2... | 1 |
| 11:27, 22 September 2018 | T--goettingen--gltT complementation.jpg (file) |  |
213 KB | Complementation of the ΔgltT mutant strain. A. The artificial Palf4 promoter (12) and the ribosome-binding site (RBS) of the B. subtilis gapA gene were fused by PCR to the gltT gene. B. Growth of the B. subtilis wild type 168 (WT), the ΔgltT mutant B... | 1 |
| 10:49, 22 September 2018 | T--goettingen--Roundup gltT gltP.jpg (file) |  |
303 KB | Growth of the strains 168 (wild type ), BP233 (ΔgltT), BP234 (ΔgltP) and BP235 (ΔgltT ΔgltP) on CS-Glc minimal medium in the presence of different amounts of glyphosate. B. Roundup® Alphee purchased from Westfalia. C. Growth of the strains 168, BP... | 1 |
| 10:19, 22 September 2018 | T--Goettingen--BBa K2586001 platereader.png (file) |  |
222 KB | Inactivation of the glutamate transporters confers high-level resistance to glyphosate. (A) Growth of the B. subtilis wild type (WT) strain 168 in CS-Glc minimal medium supplemented with increasing amounts of glyphosate (GS). (B) Growth of the Δ<i>glt... | 1 |
