User contributions
- 07:32, 21 October 2019 (diff | hist) . . (+76) . . Part:BBa K2985004 (current)
- 07:30, 21 October 2019 (diff | hist) . . (+256) . . Part:BBa K2985004
- 07:24, 21 October 2019 (diff | hist) . . (+6) . . Part:BBa K2985160 (current)
- 07:24, 21 October 2019 (diff | hist) . . (+6) . . Part:BBa K2985002 (current)
- 07:20, 21 October 2019 (diff | hist) . . (+174) . . Part:BBa K2985002
- 07:16, 21 October 2019 (diff | hist) . . (+4) . . Part:BBa K2985002
- 07:15, 21 October 2019 (diff | hist) . . (+304) . . Part:BBa K2985002
- 07:11, 21 October 2019 (diff | hist) . . (+174) . . Part:BBa K2985160
- 07:06, 21 October 2019 (diff | hist) . . (+382) . . Part:BBa K2985160
- 03:44, 20 October 2019 (diff | hist) . . (-2) . . Part:BBa K2985002
- 03:43, 20 October 2019 (diff | hist) . . (+2,603) . . Part:BBa K2985002
- 03:36, 20 October 2019 (diff | hist) . . (+97) . . N File:Jiangnan-China yerP-figure 4.png (Fig. 4 the morphology of Bacillus subtilis after transformation at higher surfactin concentration) (current)
- 03:36, 20 October 2019 (diff | hist) . . (+88) . . N File:Jiangnan-China yerP-figure 3.png (Figure 3 Morphology of wild-type Bacillus subtilis 168 at higher surfactin concentration) (current)
- 03:36, 20 October 2019 (diff | hist) . . (+4) . . N File:Jiangnan-China yerP-figure 5.jpeg (HPLC) (current)
- 03:35, 20 October 2019 (diff | hist) . . (+97) . . N File:Jiangnan-China yerP-figure 1.jpeg (Figure 1: the red gene is the yerP gene, which we inserted. Its location is between yerO and yerQ) (current)
- 03:35, 20 October 2019 (diff | hist) . . (+138) . . N File:Jiangnan-China yerP-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 3198 bp, which is consistent with the target length.) (current)
- 03:31, 20 October 2019 (diff | hist) . . (0) . . File:Jiangnan-China ycxA-figure 1.jpeg (JNUsdq uploaded a new version of File:Jiangnan-China ycxA-figure 1.jpeg) (current)
- 03:30, 20 October 2019 (diff | hist) . . (+7) . . Part:BBa K2985004
- 03:27, 20 October 2019 (diff | hist) . . (+3,433) . . Part:BBa K2985004
- 03:20, 20 October 2019 (diff | hist) . . (+88) . . N File:Jiangnan-China ycxA-figure 7.jpeg (after transformation, the colony is relatively large and the hemolysis circle is larger.) (current)
- 03:20, 20 October 2019 (diff | hist) . . (+62) . . N File:Jiangnan-China ycxA-figure 4.jpeg (co-culture of wild type bacillus subtilis and Escherichia coli) (current)
- 03:19, 20 October 2019 (diff | hist) . . (+67) . . N File:Jiangnan-China ycxA-figure 6.jpeg (relatively small colonies producing surfactin before transformation) (current)
- 03:19, 20 October 2019 (diff | hist) . . (+73) . . N File:Jiangnan-China ycxA-figure 5.jpeg (co-culture of Bacillus subtilis and Escherichia coli after transformation) (current)
- 03:18, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China ycxA-figure 3.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1227bp, consistent with the target length.) (current)
- 03:18, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China ycxA-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1104bp, consistent with the target length.) (current)
- 03:16, 20 October 2019 (diff | hist) . . (+155) . . N File:Jiangnan-China ycxA-figure 1.jpeg (Figure 1: upstream homologous arm (742bp) + SPC resistance gene (1104bp) + P43 promoter (678 / 56bp) + ycxA (1227bp) + downstream homologous arm(646bp))
- 03:05, 20 October 2019 (diff | hist) . . (-10) . . Part:BBa K322921:Experience (current)
- 03:04, 20 October 2019 (diff | hist) . . (-5) . . Part:BBa K322921:Experience
- 03:04, 20 October 2019 (diff | hist) . . (+3) . . Part:BBa K322921:Experience
- 03:01, 20 October 2019 (diff | hist) . . (+46) . . Part:BBa K322921:Experience
- 02:59, 20 October 2019 (diff | hist) . . (+5) . . N File:Jiangnan-China sacB-figure 7.jpeg (table) (current)
- 02:53, 20 October 2019 (diff | hist) . . (+20) . . Part:BBa K143013
- 02:51, 20 October 2019 (diff | hist) . . (-97) . . Part:BBa K322921:Experience
- 02:50, 20 October 2019 (diff | hist) . . (+17) . . Part:BBa K322921:Experience
- 02:48, 20 October 2019 (diff | hist) . . (+3) . . Part:BBa K322921:Experience
- 02:46, 20 October 2019 (diff | hist) . . (+3,555) . . Part:BBa K322921:Experience
- 02:39, 20 October 2019 (diff | hist) . . (+37) . . N File:Jiangnan-China sacB-figure 6.png (Enzyme activity-sucrose concentration) (current)
- 02:37, 20 October 2019 (diff | hist) . . (+41) . . N File:Jiangnan-China sacB-figure 4.jpeg (medium without expression of pullulanase) (current)
- 02:37, 20 October 2019 (diff | hist) . . (+42) . . N File:Jiangnan-China sacB-figure 5.jpeg (culture medium with pullulanase expression) (current)
- 02:36, 20 October 2019 (diff | hist) . . (+116) . . N File:Jiangnan-China sacB-figure 3.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.) (current)
- 02:36, 20 October 2019 (diff | hist) . . (+173) . . N File:Jiangnan-China sacB-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation of positive cloned DNA. The product has a signal band at 534bp respectively, which is consistent with the target length.) (current)
- 02:35, 20 October 2019 (diff | hist) . . (+80) . . N File:Jiangnan-China sacB-figure1.jpeg (sacB promoter (534bp) + RBS (BBa_K2985008) + pul gene (BBa_K2985009) + screening) (current)
- 02:26, 20 October 2019 (diff | hist) . . (+1,145) . . Part:BBa K322921:Experience
- 02:21, 20 October 2019 (diff | hist) . . (-1) . . Part:BBa K143013
- 02:17, 20 October 2019 (diff | hist) . . (+1) . . Part:BBa K143013
- 02:17, 20 October 2019 (diff | hist) . . (+62) . . N File:Jiangnan-China P43-figure10.jpeg (P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen) (current)
- 02:16, 20 October 2019 (diff | hist) . . (-12) . . Part:BBa K143013
- 02:14, 20 October 2019 (diff | hist) . . (+2,912) . . Part:BBa K143013
- 02:01, 20 October 2019 (diff | hist) . . (+20) . . N File:Jiangnan-China P43-figure 7.png (Enzyme activity-Time) (current)
- 02:01, 20 October 2019 (diff | hist) . . (+7) . . N File:Jiangnan-China P43-figure 6.png (OD-Time) (current)
- 02:00, 20 October 2019 (diff | hist) . . (+42) . . N File:Jiangnan-China P43-figure 5.jpeg (culture medium with pullulanase expression) (current)
- 01:59, 20 October 2019 (diff | hist) . . (+40) . . N File:Jiangnan-China P43-figure 4.jpeg (medium without expression of pullulanase) (current)
- 01:58, 20 October 2019 (diff | hist) . . (+116) . . N File:Jiangnan-China P43-figure 3.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.) (current)
- 01:58, 20 October 2019 (diff | hist) . . (+178) . . N File:Jiangnan-China P43-figure 2.jpeg (The positive clone DNA was identified by 0.8% agarose gel electrophoresis and PCR. The product of plasmid has a signal band at 637 BP, which is consistent with the target length.) (current)
- 01:57, 20 October 2019 (diff | hist) . . (+62) . . N File:Jiangnan-China P43-figure 1.jpeg (P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen) (current)
- 01:56, 20 October 2019 (diff | hist) . . (+1,564) . . Part:BBa K143013
- 01:53, 20 October 2019 (diff | hist) . . (0) . . Part:BBa K2985160
- 01:52, 20 October 2019 (diff | hist) . . (+111) . . N File:Jiangnan-China liaH-figure 8.jpeg (the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis) (current)
- 01:52, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China liaH-figure 7.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left) (current)
- 01:24, 20 October 2019 (diff | hist) . . (0) . . Part:BBa K2985160
- 01:23, 20 October 2019 (diff | hist) . . (0) . . Part:BBa K2985160
- 01:22, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China liaH-figure 5.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left) (current)
- 01:20, 20 October 2019 (diff | hist) . . (-22) . . Part:BBa K2985160
- 01:19, 20 October 2019 (diff | hist) . . (+22) . . Part:BBa K2985160
- 01:16, 20 October 2019 (diff | hist) . . (+2,338) . . Part:BBa K2985160
- 01:10, 20 October 2019 (diff | hist) . . (+111) . . N File:Jiangnan-China liaH-figure 4.jpeg (the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis) (current)
- 01:08, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China liaH-figure 3.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left) (current)
- 01:06, 20 October 2019 (diff | hist) . . (+128) . . N File:Jiangnan-China liaH-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 678bp, consistent with the target length.) (current)
- 01:05, 20 October 2019 (diff | hist) . . (+8) . . N File:Jiangnan-China liaH-figure 1.jpeg (P43-liaH) (current)
- 01:01, 20 October 2019 (diff | hist) . . (+388) . . Part:BBa K2985160
- 00:55, 20 October 2019 (diff | hist) . . (+2,483) . . Part:BBa K2985009 (current)
- 00:50, 20 October 2019 (diff | hist) . . (+27) . . Part:BBa K2985009
- 00:48, 20 October 2019 (diff | hist) . . (-78) . . Part:BBa K2985009
- 00:48, 20 October 2019 (diff | hist) . . (+77) . . Part:BBa K2985009
- 00:41, 20 October 2019 (diff | hist) . . (+630) . . Part:BBa K2985009
- 00:30, 20 October 2019 (diff | hist) . . (+58) . . N File:Figure 3-pul.jpeg (medium including red Pullulan with pullulanase expression) (current)
- 16:35, 19 October 2019 (diff | hist) . . (+63) . . N File:Figure 2-pul.jpeg (medium including red Pullulan without expression of pullulanase) (current)
- 16:32, 19 October 2019 (diff | hist) . . (+116) . . N File:Figure 1-pul.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.) (current)
- 04:33, 16 October 2019 (diff | hist) . . (-241) . . Part:BBa K2985120 (current)
- 07:14, 14 October 2019 (diff | hist) . . (+26) . . Part:BBa K2985000 (current)
- 07:11, 14 October 2019 (diff | hist) . . (-10) . . Part:BBa K2985000
- 16:39, 12 October 2019 (diff | hist) . . (-250) . . Part:BBa K2985002