File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 03:36, 20 October 2019 | Jiangnan-China yerP-figure 4.png (file) |  |
282 KB | Fig. 4 the morphology of Bacillus subtilis after transformation at higher surfactin concentration | 1 |
| 03:36, 20 October 2019 | Jiangnan-China yerP-figure 3.png (file) |  |
282 KB | Figure 3 Morphology of wild-type Bacillus subtilis 168 at higher surfactin concentration | 1 |
| 03:36, 20 October 2019 | Jiangnan-China yerP-figure 5.jpeg (file) |  |
49 KB | HPLC | 1 |
| 03:35, 20 October 2019 | Jiangnan-China yerP-figure 1.jpeg (file) |  |
9 KB | Figure 1: the red gene is the yerP gene, which we inserted. Its location is between yerO and yerQ | 1 |
| 03:35, 20 October 2019 | Jiangnan-China yerP-figure 2.jpeg (file) |  |
22 KB | 0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 3198 bp, which is consistent with the target length. | 1 |
| 03:31, 20 October 2019 | Jiangnan-China ycxA-figure 1.jpeg (file) |  |
10 KB | Figure 1: upstream homologous arm (742bp) + SPC resistance gene (1104bp) + P43 promoter (678 / 56bp) + ycxA (1227bp) + downstream homologous arm（646bp） | 2 |
| 03:20, 20 October 2019 | Jiangnan-China ycxA-figure 7.jpeg (file) |  |
44 KB | after transformation, the colony is relatively large and the hemolysis circle is larger. | 1 |
| 03:20, 20 October 2019 | Jiangnan-China ycxA-figure 4.jpeg (file) |  |
72 KB | co-culture of wild type bacillus subtilis and Escherichia coli | 1 |
| 03:19, 20 October 2019 | Jiangnan-China ycxA-figure 6.jpeg (file) |  |
43 KB | relatively small colonies producing surfactin before transformation | 1 |
| 03:19, 20 October 2019 | Jiangnan-China ycxA-figure 5.jpeg (file) |  |
64 KB | co-culture of Bacillus subtilis and Escherichia coli after transformation | 1 |
| 03:18, 20 October 2019 | Jiangnan-China ycxA-figure 3.jpeg (file) |  |
11 KB | 0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1227bp, consistent with the target length. | 1 |
| 03:18, 20 October 2019 | Jiangnan-China ycxA-figure 2.jpeg (file) |  |
14 KB | 0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1104bp, consistent with the target length. | 1 |
| 02:59, 20 October 2019 | Jiangnan-China sacB-figure 7.jpeg (file) |  |
22 KB | table | 1 |
| 02:39, 20 October 2019 | Jiangnan-China sacB-figure 6.png (file) |  |
412 KB | Enzyme activity-sucrose concentration | 1 |
| 02:37, 20 October 2019 | Jiangnan-China sacB-figure 4.jpeg (file) |  |
87 KB | medium without expression of pullulanase | 1 |
| 02:37, 20 October 2019 | Jiangnan-China sacB-figure 5.jpeg (file) |  |
77 KB | culture medium with pullulanase expression | 1 |
| 02:36, 20 October 2019 | Jiangnan-China sacB-figure 3.jpeg (file) |  |
13 KB | An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase. | 1 |
| 02:36, 20 October 2019 | Jiangnan-China sacB-figure 2.jpeg (file) |  |
8 KB | 0.8% agarose gel electrophoresis and PCR validation of positive cloned DNA. The product has a signal band at 534bp respectively, which is consistent with the target length. | 1 |
| 02:35, 20 October 2019 | Jiangnan-China sacB-figure1.jpeg (file) |  |
26 KB | sacB promoter (534bp) + RBS (BBa_K2985008) + pul gene (BBa_K2985009) + screening | 1 |
| 02:17, 20 October 2019 | Jiangnan-China P43-figure10.jpeg (file) |  |
27 KB | P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen | 1 |
| 02:01, 20 October 2019 | Jiangnan-China P43-figure 7.png (file) |  |
79 KB | Enzyme activity-Time | 1 |
| 02:01, 20 October 2019 | Jiangnan-China P43-figure 6.png (file) |  |
429 KB | OD-Time | 1 |
| 02:00, 20 October 2019 | Jiangnan-China P43-figure 5.jpeg (file) |  |
83 KB | culture medium with pullulanase expression | 1 |
| 01:59, 20 October 2019 | Jiangnan-China P43-figure 4.jpeg (file) |  |
98 KB | medium without expression of pullulanase | 1 |
| 01:58, 20 October 2019 | Jiangnan-China P43-figure 3.jpeg (file) |  |
12 KB | An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase. | 1 |
| 01:58, 20 October 2019 | Jiangnan-China P43-figure 2.jpeg (file) |  |
15 KB | The positive clone DNA was identified by 0.8% agarose gel electrophoresis and PCR. The product of plasmid has a signal band at 637 BP, which is consistent with the target length. | 1 |
| 01:57, 20 October 2019 | Jiangnan-China P43-figure 1.jpeg (file) |  |
75 KB | P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen | 1 |
| 01:52, 20 October 2019 | Jiangnan-China liaH-figure 8.jpeg (file) |  |
112 KB | the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis | 1 |
| 01:52, 20 October 2019 | Jiangnan-China liaH-figure 7.jpeg (file) |  |
160 KB | the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left | 1 |
| 01:22, 20 October 2019 | Jiangnan-China liaH-figure 5.jpeg (file) |  |
263 KB | the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left | 1 |
| 01:10, 20 October 2019 | Jiangnan-China liaH-figure 4.jpeg (file) |  |
845 KB | the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis | 1 |
| 01:08, 20 October 2019 | Jiangnan-China liaH-figure 3.jpeg (file) |  |
1.04 MB | the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left | 1 |
| 01:06, 20 October 2019 | Jiangnan-China liaH-figure 2.jpeg (file) |  |
9 KB | 0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 678bp, consistent with the target length. | 1 |
| 01:05, 20 October 2019 | Jiangnan-China liaH-figure 1.jpeg (file) |  |
13 KB | P43-liaH | 1 |
| 00:30, 20 October 2019 | Figure 3-pul.jpeg (file) |  |
48 KB | medium including red Pullulan with pullulanase expression | 1 |
| 16:35, 19 October 2019 | Figure 2-pul.jpeg (file) |  |
53 KB | medium including red Pullulan without expression of pullulanase | 1 |
| 16:32, 19 October 2019 | Figure 1-pul.jpeg (file) |  |
12 KB | An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase. | 1 |
