All public logs

Combined display of all available logs of parts.igem.org. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 03:36, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China yerP-figure 4.png (Fig. 4 the morphology of Bacillus subtilis after transformation at higher surfactin concentration)
• 03:36, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China yerP-figure 3.png (Figure 3 Morphology of wild-type Bacillus subtilis 168 at higher surfactin concentration)
• 03:36, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China yerP-figure 5.jpeg (HPLC)
• 03:35, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China yerP-figure 1.jpeg (Figure 1: the red gene is the yerP gene, which we inserted. Its location is between yerO and yerQ)
• 03:35, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China yerP-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 3198 bp, which is consistent with the target length.)
• 03:31, 20 October 2019 JNUsdq (Talk | contribs) uploaded a new version of File:Jiangnan-China ycxA-figure 1.jpeg (Figure 1: upstream homologous arm (742bp) + SPC resistance gene (1104bp) + P43 promoter (678 / 56bp) + ycxA (1227bp) + downstream homologous arm（646bp）)
• 03:20, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 7.jpeg (after transformation, the colony is relatively large and the hemolysis circle is larger.)
• 03:20, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 4.jpeg (co-culture of wild type bacillus subtilis and Escherichia coli)
• 03:19, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 6.jpeg (relatively small colonies producing surfactin before transformation)
• 03:19, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 5.jpeg (co-culture of Bacillus subtilis and Escherichia coli after transformation)
• 03:18, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 3.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1227bp, consistent with the target length.)
• 03:18, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 1104bp, consistent with the target length.)
• 03:16, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China ycxA-figure 1.jpeg (Figure 1: upstream homologous arm (742bp) + SPC resistance gene (1104bp) + P43 promoter (678 / 56bp) + ycxA (1227bp) + downstream homologous arm（646bp）)
• 02:59, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 7.jpeg (table)
• 02:39, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 6.png (Enzyme activity-sucrose concentration)
• 02:37, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 4.jpeg (medium without expression of pullulanase)
• 02:37, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 5.jpeg (culture medium with pullulanase expression)
• 02:36, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 3.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.)
• 02:36, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation of positive cloned DNA. The product has a signal band at 534bp respectively, which is consistent with the target length.)
• 02:35, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China sacB-figure1.jpeg (sacB promoter (534bp) + RBS (BBa_K2985008) + pul gene (BBa_K2985009) + screening)
• 02:17, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure10.jpeg (P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen)
• 02:01, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 7.png (Enzyme activity-Time)
• 02:01, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 6.png (OD-Time)
• 02:00, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 5.jpeg (culture medium with pullulanase expression)
• 01:59, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 4.jpeg (medium without expression of pullulanase)
• 01:58, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 3.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.)
• 01:58, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 2.jpeg (The positive clone DNA was identified by 0.8% agarose gel electrophoresis and PCR. The product of plasmid has a signal band at 637 BP, which is consistent with the target length.)
• 01:57, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China P43-figure 1.jpeg (P43 promoter (56bp) + pullulanase gene (bba_k2985009) + screen)
• 01:52, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 8.jpeg (the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis)
• 01:52, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 7.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left)
• 01:22, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 5.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left)
• 01:10, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 4.jpeg (the left is the normal LB medium, and the right contains 200mg/L of surfactin of the modified bacillus subtilis)
• 01:08, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 3.jpeg (the growth of wild-type bacillus subtilis containing 200mg/L of surfactin is shown on the right and normal LB medium on the left)
• 01:06, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 2.jpeg (0.8% agarose gel electrophoresis and PCR validation. The product has a signal band at 678bp, consistent with the target length.)
• 01:05, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Jiangnan-China liaH-figure 1.jpeg (P43-liaH)
• 00:30, 20 October 2019 JNUsdq (Talk | contribs) uploaded File:Figure 3-pul.jpeg (medium including red Pullulan with pullulanase expression)
• 16:35, 19 October 2019 JNUsdq (Talk | contribs) uploaded File:Figure 2-pul.jpeg (medium including red Pullulan without expression of pullulanase)
• 16:32, 19 October 2019 JNUsdq (Talk | contribs) uploaded File:Figure 1-pul.jpeg (An additional protein band was added near 100 kDa and was in line with the expected molecular weight of pullulanase.)