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Part:BBa_K863206

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)
Revision as of 03:37, 27 October 2012 by JSchirmacher (Talk | contribs)

Mating factor alpha 1 with Kozak sequence

With a N-terminal mating factor alpha 1 from Saccharomyces sereviceae linked to the protein of interesst, it could secreted into the media. This allows a better purification of the protein.

This part is a part of a shuttle vector (K863204) that could use for site directed recombination in the yeast P. pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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