Part:BBa_K4162006

T7_RBS

contributed by Fudan iGEM 2023

Fudan iGEM 2023

This is an efficient ribosome binding site for constitutive protein translation, from bacteriophage T7 gene 10 ^[1]^[2]. We have used it in 2022 as the RBS that drives intermediate-level protein expression. Using T7_RBS gives us an option to switch to a weaker J6_RBS BBa_J61100 or a stronger B0_RBS BBa_B0030, which have been successfully demonstrated last year BBa_K4162117 and BBa_K4162118. We continue this strategy in 2023.

Introduction

T7 ribosome binding site (RBS) was discovered from the T7 phage genome, commonly used to drive protein expression in bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. Olins PO, Rangwala SH. J Biol Chem, 1989 Oct 15;264(29):16973-6. PMID:2676996
↑ The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Olins PO, Devine CS, Rangwala SH, Kavka KS. Gene, 1988 Dec 15;73(1):227-35. PMID:3072257

[1] A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli. Olins PO, Rangwala SH. J Biol Chem, 1989 Oct 15;264(29):16973-6. PMID:2676996

[2] The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Olins PO, Devine CS, Rangwala SH, Kavka KS. Gene, 1988 Dec 15;73(1):227-35. PMID:3072257

[1]

[2]