Coding
EforRed
Part:BBa_K3892006
Designed by: iGEM21_Linkoping_Sweden Group: iGEM21_Linkoping_Sweden (2021-10-17)
EforRed
Red chromoprotein with strong RBS. This is iGEM21_Linkoping_Sweden's improvement of the BBa_K1073022. The BioBrick was improved by adding a stronger RBS and spacers (G-10 leader, BBa_K1758100 but the polyA sequence were removed and turned into BBa_K3892010) which increases the expression of the protein. This version of the protein has a glycine added after the start methionine to create a BamHI restriction enzyme site. This enables swapping of the RBS and promotor parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/chromoprotein
None |