Coding
EforRed

Part:BBa_K3892006

Designed by: iGEM21_Linkoping_Sweden   Group: iGEM21_Linkoping_Sweden   (2021-10-17)
Revision as of 08:18, 21 October 2021 by Olle111 (Talk | contribs)


EforRed

Red chromoprotein with strong RBS. This is iGEM21_Linkoping_Sweden's improvement of the BBa_K1073022. The BioBrick was improved by adding a stronger RBS and spacers (G-10 leader, BBa_K1758100 but the polyA sequence were removed and turned into BBa_K3892010) which increases the expression of the protein. This version of the protein has a glycine added after the start methionine to create a BamHI restriction enzyme site. This enables swapping of the RBS and promotor parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
Parameters
None