Difference between revisions of "Part:BBa K2656126"
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| − | <li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version | + | <li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version. <html></li> |
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version <html></li> | <li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version <html></li> | ||
<li></html> [https://parts.igem.org/Part:BBa_K2656025 BBa_K2656025]: the [https://parts.igem.org/Part:BBa_J45004 BBa_J45004] BMSTI coding sequence in its Golden Braid standardized version <html></li> | <li></html> [https://parts.igem.org/Part:BBa_K2656025 BBa_K2656025]: the [https://parts.igem.org/Part:BBa_J45004 BBa_J45004] BMSTI coding sequence in its Golden Braid standardized version <html></li> | ||
Latest revision as of 21:31, 17 October 2018
BMSTI transcriptional unit
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version.
- BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version
- BBa_K2656025: the BBa_J45004 BMSTI coding sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 728
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]