Plasmid_Backbone
Transfecti

Part:BBa_K157041

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)
Revision as of 03:38, 9 December 2009 by Rshetty (Talk | contribs)

Transfection vector (similar to pSB1AK8)

Transfectionvector with CMV promoter and CFP as reporter. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

  • RS 23:57, 8 December 2009 (UTC): This part has been split into two parts: a plasmid backbone (this part, BBa_K157041) and a part (the insert, BBa_K157043). The physical DNA needs to be remapped to BBa_K157041-K157043.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


[edit]
Categories
//chassis/eukaryote
//plasmidbackbone/operation
Parameters
chassis
copies
originpMB1
resistanceAK