Part:BBa_K157041

Transfection vector (similar to pSB1AK8)

Transfectionvector with CMV promoter and CFP as reporter. This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

• RS 23:57, 8 December 2009 (UTC): This part has been split into two parts: a plasmid backbone (this part, BBa_K157041) and a part (the insert, BBa_K157045). The physical DNA needs to be remapped to BBa_K157041-K157045.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 3405
  Illegal XbaI site found at 3420
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3411
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal XhoI site found at 1272
  Illegal XhoI site found at 2298
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3405
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3420
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2444