Part:BBa_K1184000:Experience

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UNIQb7d829d24c37fc8e-partinfo-00000000-QINU

••• Carnegie_Mellon 2013

Figure 1: Phototoxicity of KillerRed and mRFP1 (BBa_E1010) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours. KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1. RFP 114% ± 20% (viable cells)/(Normalized RFU) KillerRed: 53% ± 17% (viable cells)/(Normalized RFU) Figure 1: Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp XL10 Ultracompetent cells were transformed with BBa_K1184000 cloned with BBa_B0034 as the RBS and BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2. Table 1 Tecan Safire2 Parameters Excitation (nm) 585 Emission (nm) 610 Excitation bandwidth (nm) 10 Emission bandwidth (nm) 10 Gain 129 Number of reads 10 Integration Time (microseconds) 40 Table 2 Shows the fluorescence data over time during photobleaching. Time (minutes) Fluorescence (RFU) 0 42598 20 37616 40 33749 60 29059 80 25680 100 21985 120 19442 140 17031 160 15738 180 13741

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