Reporter

Part:BBa_K1073024

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 14:03, 27 September 2013 by Laura S (Talk | contribs) (→‎Usage and Biology)

Constitutively expressed chromoprotein amilGFP

The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.


Usage and Biology

During cultivation the chromoprotein amilGFP is expressed and stored intracellular. The yellow color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter or selection marker and seperate cells easily.

Expressed chromoproteins.png AmilGFP pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing amilGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None