Device
pBAD-CDE-D

Part:BBa_K323132:Design

Designed by: Tina Lebar, Tjaa Stoicki   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 03:55, 27 October 2010 by TinaL (Talk | contribs) (→‎Source)

VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3078
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 4320
    Illegal SapI.rc site found at 4395


Design Notes

Part:BBa_K323087 was ligated into a vector containing Part:BBa_K323086 as a back insert. Part:BBa_K323131 was later ligated into the resulting BioBrick as a back insert. The fragment containing the latter three BioBrick parts was then ligated into the BsaI cloning site of Part:BBa_K323110.

Source

The part was designed from four parts (Part:BBa_K323110, Part:BBa_K323087, Part:BBa_K323086 and Part:BBa_K323131) that our team previously constructed.

References