Composite

Part:BBa_K338003:Design

Designed by: Caltech iGEM 2010   Group: iGEM10_Caltech   (2010-10-26)
Revision as of 10:57, 26 October 2010 by Registry (Talk | contribs)

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PHA Synthase Composite, Part 1/2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 338
    Illegal AgeI site found at 701
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the BBa_K215000 promoter. Naturally, each gene is preceded by a standard RBS (BBa_B0034) and the transcript finishes with a strong terminator (BBa_B0015), for a total size of about 3500bp.

Note that these three genes should only cause the production of PHB oligomers in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the phaC1 gene could cause some crosslinking, but this has not been experimentally verified.


Source

Registry of Standard Biological Parts

References