Generator
Part:BBa_K215000
Designed by: Chris Eiben Group: iGEM09_Washington (2009-09-30)
R0011+B0034, strong IPTG-inducible promoter with strong RBS.
This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. This part is in pSB1A3, which has a built in transcriptional terminator located 3' of the insertion site for coding sequences. This allows for a protein coding sequence to be inserted into this vector and be immediately ready for expression.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//plasmidbackbone/expression/inducible
n/a | R0011+B0034, strong IPTG-inducible promoter with strong RBS. |