Part:BBa_K389421:Design

VirA/G signaling system + sensitivity tuner + luciferase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 647
  Illegal NheI site found at 2581
  Illegal NheI site found at 2604
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1632
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 5002
  Illegal NgoMIV site found at 6346
  Illegal NgoMIV site found at 6367
  Illegal AgeI site found at 4525
  Illegal AgeI site found at 4637
  Illegal AgeI site found at 4839
  Illegal AgeI site found at 6070
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1768
  Illegal SapI.rc site found at 6252
  

Design Notes

a modified sensitivity tuner BBa_I746370 to enhance the luciferase signal which interacts as a reporter for a VirA/G signaling system

Source

• luciferase from pGL4.10luc2 (Promega) (BBa_K389004)
• terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
• sensitivity tuner part consists inter alia of
  • ogr activator from phage P2 (BBa_I746350)
  • PF promoter from phage P2 (BBa_I746360)
• virG was synthesized by Mr. Gene (BBa_K389002)
• virA and vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389001, BBa_K389003)

References

[http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]