Composite

Part:BBa_K389421:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

VirA/G signaling system + sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5002
    Illegal NgoMIV site found at 6346
    Illegal NgoMIV site found at 6367
    Illegal AgeI site found at 4525
    Illegal AgeI site found at 4637
    Illegal AgeI site found at 4839
    Illegal AgeI site found at 6070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768
    Illegal SapI.rc site found at 6252


Design Notes

a modified sensitivity tuner BBa_I746370 to enhance the luciferase signal which interacts as a reporter for a VirA/G signaling system

Source

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]