Part:BBa_K157040

Eukaryotic terminator vector (very similar to pSB1AK8)

This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

It is derived from BBa_J52017. There are a couple differences. It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10). It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).

• RS 19:26, 23 November 2008 (UTC): This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042. BBa_K157040 is the plasmid backbone and BBa_K157042 is the part. The physical DNA needs to be remapped to BBa_K157042.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal XbaI site found at 3420
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 8
  Illegal NotI site found at 3411
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal XhoI site found at 1272
  Illegal XhoI site found at 2298
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a suffix.
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3405
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3420
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2444