Part:pSB1AK8

Eukaryotic terminator vector (formerly BBa_J52017)

This vector is BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

• RS 03:20, 9 December 2009 (UTC): This plasmid backbone is not fully compliant with Assembly standard 10. It is missing a C in the BioBrick suffix. The NotI and PstI sites overlap as the sequence is currently specified in the Registry. It is not known whether this error is simply a user entry error. Or if the deletion is present in the physical DNA as well.

• RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now pSB1AK8-BBa_P1010.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3411
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3405
  Illegal XhoI site found at 1272
  Illegal XhoI site found at 2298
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3405
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3405
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3420
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2444