Composite
Part:BBa_K5117048
Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann Group: iGEM24_TU-Dresden (2024-09-28)
PcotYZ-BsRBS-AtCelO-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- celO gene of Acetivibrio thermocellus without signal peptide encoding an exoglucanase (EC 3.2.1.176),
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 href="https://parts.igem.org/Part:BBa_K5117047">(BBa_K5117047)
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of AtCelO on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellulose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1184
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2170
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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