Composite

Part:BBa_K5117048

Designed by: Jenny Sauermann   Group: iGEM24_TU-Dresden   (2024-09-28)


PcotYZ-BsRBS-AtCelO-L2-CotY-B0014

This part serves as transcriptional unit composed of:

- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),

- ribosome binding site of Bacillus subtilis (BBa_K5117000),

- celO gene of Acetivibrio thermocellum without signal peptide encoding an exoglucanase (EC 3.2.1.176),

addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117047),

- cotY gene of Bacillus subtilis (BBa_K5117022),

- bidirectional terminator B0014 (BBa_B0014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2170
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None