rocR, PA3947, cyclic di-GMP phosphodiesterase

Description

Degradation of c-di-GMP in Gluconacetobacter hansenii ATCC 53582.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 258
Illegal BamHI site found at 1171
Illegal XhoI site found at 94
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 895
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 802

2021 SZPT-China

Biology

The gene rocR is originated from the genome of Pseudomonas aeruginosa (PAO1). And RocR expressed by rocR is the phosphodiesterase (PDE) of c-di-GMP.

Usage

The encoding sequences for RocR were inserted into the expression vector with BBa_K880005 (BBa_J23100 & BBa_B0034) to obtain J23100-B0034-rocR-rrnB T1 (BBa_K3740065). We introduced the constructed plasmid into E. coli DH5α to verify its successful construction, and then transferred it into G. hansenii ATCC 53582 to verify its function.

Figure 1. Gene circuit of rocR

Characterization

1. Identification

<p>As shown in Figure 2, c-di-GMP phosphodiesterase-encoded genes BBa_K3740065 was identified successfully by PCR amplification.