Coding

Part:BBa_K3740022

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-26)
Revision as of 13:36, 18 October 2021 by HUANG (Talk | contribs) (→‎2021 SZPT-China)


fcsR, PA2133, cyclic di-GMP phosphodiesterase

Description

Degradation of c-di-GMP in Gluconacetobacter hansenii ATCC 53582.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 703
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 806
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 700
    Illegal BsaI.rc site found at 448
    Illegal SapI.rc site found at 813


2021 SZPT-China

Biology

fcsR is originated from the genome of Pseudomonas aeruginosa (PAO1), the FcsR expressed by fcsR is the phosphodiesterase (PDE) of c-di-GMP.

Usage

The encoding sequences for FcsR were inserted into the expression vector with BBa_K880005 (BBa_J23100 & BBa_B0034) to obtain J23100-B0034-fcsR-rrnB T1 (BBa_K3740062). We introduced the constructed plasmid into E. coli DH5α to verify its successful construction, and then transferred it into G. hansenii ATCC 53582 to verify its function.

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