Part:pSB1AC3

High copy BioBrick assembly plasmid

pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AC3

pSB1AC3 Plasmid map

Usage and Biology

• Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)

• Chloramphenicol resistant up to 800 ug/ml - tested with E. coli DH5alpha according to the standardized procedure (Wiegand et al., Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 3(2), 163-175, 2008)

• Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

• This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18901

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3034
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3040
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3034
  Illegal XhoI site found at 1035
  Illegal XhoI site found at 1927
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3034
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3034
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3049
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2073