Plasmid_Backbone
Part:pSB1AC3:Design
Designed by: Tom Knight Group: iGEM05_Registry_Example (2005-06-07)
High copy BioBrick assembly plasmid
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3040 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal XhoI site found at 1035
Illegal XhoI site found at 1927 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3034
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3034
Plasmid lacks a suffix.
Illegal XbaI site found at 3049
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2073
Design Notes
Source
pSB1A3 with added Cm resistance