Composite
Part:BBa_K3484003:Design
Designed by: Eduard Sune & Andreu Pascuet Group: iGEM20_UPF_Barcelona (2020-10-26)
Revision as of 20:50, 27 October 2020 by Andreupf99 (Talk | contribs)
Intein-mediated T3(THRB) → eGFP (transcriptional unit)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 235
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 725
Illegal NgoMIV site found at 2228 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1640
Design Notes
Special considerations have been taken into account for the construction of the protein domain and can be seen at the registry of the basic part BBa_K3484000.
Source
Parts used for this composite are all from previous entries of the iGEM Registry:
- Promoter (BBa_J23100) + Ribosome Binding Site (BBa_B0034): BBa_K880005
- Protein domain (intein-mediated T3 biosensor): BBa_K3484000
- Double terminator (BBa_B0012 -BBa_B0011): BBa_B0014
References
Look for references at BBa_K3484000.