Signalling

Part:BBa_K3411040

Designed by: Janet Standeven, Nikhita Lalwani, Sindhura Mettupalli, Kathy Ye, Sachintha Ashok, Saif Khan   Group: iGEM20_Lambert_GA   (2020-10-23)
Revision as of 00:25, 27 October 2020 by Msindhu01824 (Talk | contribs)


NarL Gene

Lambert_GA iGEM's NarL system is modeled after E.coli’s natural nitrite and nitrate sensor, the Nar Operon, which includes the NarL response regulator, the gene listed above, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects both nitrite and nitrate. This specific part is the isolated NarL gene.

The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, BBa_K1682018) NarL System serves to accept the phosphate group from NarX. The promoter BBa_J23106, a constitutive Anderson Promoter, initiates the production of TetR. TetR represses the PtetO promoter, producing NarL. In the presence of nitrate, NarX phosphorylates NarL. Therefore, phosphorylated NarL measures nitrate levels. By using the corrected difference, our NarP (Lambert_GA iGEM’s NarP composite part BBa_K3411010) and NarL composite parts can evaluate nitrate levels.

Lambert_GA iGEM will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal BglII site found at 342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None