Composite
Part:BBa_K3110040:Design
Designed by: Mrugank Dake, Amartya Pal Group: iGEM19_IISER_Tirupati (2019-10-14)
lldRO1-J23117-lldRO2 Strong RBS sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 174
Design Notes
This part contains:
- The Promoter and Operator Region: lldRO1-J23117-lldRO2 (Part:BBa_K1847008) -designed by iGEM team of ETH Zurich -2015
- A strong RBS: (Part:BBa_B0034)
- Reporter Protein: sfGFP (Part:BBa_I746916)
- Double terminator: (Part:BBa_I746916)
Source
sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.
References
- parts.igem.org/Part:BBa_K1847008
- Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006