Generator

Part:BBa_K3187044

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-13)
Revision as of 08:12, 20 October 2019 by PeterGockel (Talk | contribs)


P22 Bacteriophag Coat Protein (T7 promotor)

This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a 

                   href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a
                   href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1499
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 98
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1499
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None