Generator
Part:BBa_K3187043
Designed by: iGEM TU_Darmstadt 2019 Group: iGEM19_TU_Darmstadt (2019-10-13)
Superfolder GFP + P22 Bacteriophage Scaffolding Protein Generator (tetR/tetA promotor)
This part is a generator for the P22 bacteriophage scaffold protein fused to a super folder GFP. This part was used together with BBa_K3187044 for the dual expression of the P22 scaffold protein and the P22 coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins to the surface of P22 Virus-like particles. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 76
Illegal PstI site found at 1334 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 76
Illegal NheI site found at 1339
Illegal PstI site found at 1334 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 76
Illegal BamHI site found at 796 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 76
Illegal PstI site found at 1334 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 76
Illegal PstI site found at 1334
Illegal NgoMIV site found at 1185 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 94
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Categories
Parameters
None |