Generator
Part:BBa_K3187044
Designed by: iGEM TU_Darmstadt 2019 Group: iGEM19_TU_Darmstadt (2019-10-13)
P22 Bacteriophag Coat Protein (T7 promotor)
This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins. This part was used together with BBa_K3187043 for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1499
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 98
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1499
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1499
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |