Composite

Part:BBa_K2934004:Design

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)
Revision as of 16:34, 17 October 2019 by NirLitver (Talk | contribs) (→‎Design Notes)


AmyE - Glucose Oxidase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 487
    Illegal BsaI.rc site found at 433
    Illegal BsaI.rc site found at 1258


Design Notes

The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].

Source

GOx - BBa_K2934000

AmyE signal peptide - BBa_K2273023

linker - BBa_K2934005

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system