Composite

Part:BBa_K2934004:Design

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)
Revision as of 16:33, 17 October 2019 by NirLitver (Talk | contribs) (→‎Source)


AmyE - Glucose Oxidase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 487
    Illegal BsaI.rc site found at 433
    Illegal BsaI.rc site found at 1258


Design Notes

Source

GOx - BBa_K2934000

AmyE signal peptide - BBa_K2273023

linker - BBa_K2934005


The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (B. subtilis Secretory Protein Expression System) [1].

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system