Composite
Part:BBa_K3044023
Designed by: Catharina Bang Jensen Group: iGEM19_SDU-Denmark (2019-10-07)
dCas9 with constitutive active promotor
This dCas9 is a codon optimized version of BBa_K1150000 for expression in E. coli. Together with a single guide RNA (sgRNA), the protein can be used for silencing of specific target genes. dCas9 is the inactive form of the endonuclease Cas9. In contrast to Cas9, dCas9 does not cleave the DNA. Instead, it sterically blocks the elongation of transcription as a result of the sgRNA-DNA interaction. This will only repress the expression of the target gene thus avoiding the destruction of the gene. [1]
The dCas9 is asembled with the constitutive active promoter BBa_J23100, the RBS BBa_B0034 and the terminator BBa_B0010.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 312
Illegal BglII site found at 1386 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |