Composite
Kai 2

Part:BBa_K2623011

Designed by: Gezhi Xiao   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 02:18, 14 October 2018 by Bb1984 (Talk | contribs) (→‎SUMMARY)


Phosphorylation pathsway builder of Kai

Summary

The sequence encoding for the gene is preceded by a promoter BBa_J23100, an RBS BBa_B0034, BBa_K2623004, BBa_K2623005, BBa_K2623006 and followed by a double terminator BBa_B0015 in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.

Fig.1

































Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
    Illegal BglII site found at 3666
    Illegal BglII site found at 3735
    Illegal BamHI site found at 2248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None