Project
WOBS

Part:BBa_J45700

Designed by: MIT iGEM 2006   Group: iGEM06_MIT   (2006-10-30)
Revision as of 23:46, 8 March 2008 by Rshetty (Talk | contribs)

Wintergreen odor biosynthetic system

We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.

File:BBa J45700

Usage and Biology

To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (BBa_J45320) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1689
    Illegal NgoMIV site found at 2093
    Illegal NgoMIV site found at 2218
    Illegal NgoMIV site found at 2229
    Illegal NgoMIV site found at 2510
    Illegal NgoMIV site found at 2978
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 285


[edit]
Categories
//function/odor
s
Parameters
None