Part:BBa_J45017:Design

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 307
  Illegal NgoMIV site found at 711
  Illegal NgoMIV site found at 836
  Illegal NgoMIV site found at 847
  Illegal NgoMIV site found at 1128
  Illegal NgoMIV site found at 1596
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PchA (isochorismate synthase) catalyzes the conversion of chorismate to isochorismate. PchB (isochorismate-pyruvate lyase) catalyzes the conversion of isochorismate to salicylate.

Co-expression of the two proteins from one BioBrick part successfully generates salicylate in E. coli. For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2006 wiki].

Source

PchB and PchA are found in Pseudomonas aeruginosa. The pME3366 plasmid from which BBa_J45017 was constructed was kindly sent to the MIT 2006 iGEM team by Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne. Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].

References

Original paper describing pchBA Serino-MolGenGenet-1995. Information about the enzyme encoded by PchA Gaille-JBC-2003.

<biblio>

1. Serino-MolGenGenet-1995 pmid=7500944
2. Serino-JBacteriol-1997 pmid=8982005
3. Gaille-JBC-2002 pmid=11937513
4. Gaille-JBC-2003 pmid=12624097
5. Zaitseva-JBC-2006 pmid=16914555

</biblio>