Regulatory
p(osmY)

Part:BBa_J45993:Design

Designed by: Stephen Payne   Group: iGEM06_MIT   (2006-07-11)
Revision as of 00:02, 3 February 2008 by Rshetty (Talk | contribs) (References)


Minimal stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'

Total Length: 49 bp

Annealing Length: 21 bp

GC Content: 38.1%

Melting Temperature: 49.5 degrees C

hairpin deltaG: -2.66 kcal/mol

self dimer deltaG: -99.97 kcal/mol

Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'

Total Length: 54 bp

Annealing Length: 25 bp

GC Content: 20.0%

Melting Temperature: 46.4 degrees C

hairpin deltaG: -2.86 kcal/mol

self dimer deltaG: -99.44 kcal/mol

heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol


Source

This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.

References

<biblio>

  1. Yim pmid=8282684
  2. Schellhorn pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.