Regulatory
p(osmY)
Part:BBa_J45993:Design
Designed by: Stephen Payne Group: iGEM06_MIT (2006-07-11)
Minimal stationary phase osmY promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- Constructed by primer annealing and extension
Template
None.
Forward Primer
5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'
Reverse Primer
5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'
Source
- Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])
References
<biblio>
- Yim-1994 pmid=8282684
- Schellhorn-1998 pmid=9829938
- Wise pmid=8631665
- Funabashi pmid=11879714
- Makinoshima pmid=11985708
- Yim-1992 pmid=1317380
- Hengge-Aronis-1993 pmid=8416901
- Vijaykumar-2004 pmid=15576800
</biblio>
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.