Part:BBa_J45009

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

• Very important note: None of the indicated base changes were made. Please consult design page to determine how we worked with this part.

This part catalyzes the second step in isoamyl alcohol biosynthesis. It converts alpha-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. 3-methylbutanal is then reduced via a alcohol dehydrogenase to isoamyl alcohol (the precursor to isoamyl acetate or banana scent).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 1000
  COMPATIBLE WITH RFC[1000]