Part:BBa_J45009

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Usage and Biology

• THI3 is formerly known as KID1.
• 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).

• Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. It can only be used as a suffix part in BioBrick assembly.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3
  Illegal EcoRI site found at 904
  Illegal EcoRI site found at 1603
  Illegal EcoRI site found at 1789
  Illegal SpeI site found at 697
  
• 1000
  COMPATIBLE WITH RFC[1000]