Part:BBa_E0040:Experience
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how you used this part and how it worked out.
Applications of BBa_E0040
User Reviews
UNIQ0c551fba0fb837b8-partinfo-00000000-QINU
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USTC_China iGEM13 |
In USTC_China iGEM13's project,we bulit the N-terminal TD1 modified GFP to make it is capable of transporting through skin barrier, then induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ultraviolet ultraviolet spectrometry. Students in Wen lab validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040 ). ReferenceSUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4 |
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KAIST_iGEM_2012 |
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
UNIQ0c551fba0fb837b8-partinfo-00000009-QINU
E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.
iGEM11_WHU_China |
This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks. |
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iGEM Dundee 2013 |
The function of this part was characterised and quantified in a new salt-responsive device. The GFP encoded by BBa_E0040 was hooked up to an OmpR-responsive promoter (BBa_R0083) via a ribosome binding site (BBa_B0034) to produce a new composite part (a reporter) (BBa_K1012005). GFP fluorescence could be detected in an E. coli chassis, and we used an envZ mutant strain to show the sensor was working correctly. |
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iGEM Brasil-USP 2015 |
Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site. |
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iGEM Tianjin 2015 |
Tianjin 2015 made a fusion protein using GFP with hydrophobin sJanus to make a novel and efficient protein extraction system, and the part is BBa_K1582004. It is used to test the efficiency of Janus-based protein extraction system and provide the basic data for model to be used by more proteins. This provides a new way to purify GFP and other proteins. |
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CLSB-UK We used this part as a reporter protein in a cell free system. We found that there was observable fluorescence from this part and experienced no issues with expressing it in a cell free system. |