Part:pSB3K1

No part name specified with partinfo tag.

Plasmid pACYC177BB1 (Fig. 1) is based on pACYC177 (Fig. 2), a low-copy cloning vector (10-12 copies per cell) that confers ampicillin (ApR) and kanamycin (KmR) resistance and harbours the p15A origin of replication.

Fig. 1: Map of the pCK01BB1 plasmid

Fig. 2: Map of the original pACYC177 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pACYC177 was digested at its BamHI and PstI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. However, this leads to a loss of the original ampicillin resistance and only kanamycin resistance is left. The term "BB1" in pACYC177BB1 refers to "BioBrick version 1".

Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 6, Part 7, Part 10, Part 11 and the composites BBa_I739018, BBa_I739019 and BBa_I739020. More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here].

Sequence and Features No part name specified with partinfo tag.