Part:BBa_I739007

Double regulated expression cassette for P22 cII +LVA (I739105.B0034.C0053.B0015)

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) under control of the double promoter BBa_I739105 followed by the ribosome binding site BBa_B0034. The transcription of cI is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII production can be induced by LuxR/HSL and repressed by cI. There is no inducer available which neutralizes the action of cI.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the learning part of the system. In the project description, this part is also termed Part 7. The synthesized P22 cII interacts with the double promoters BBa_I739104, BBa_I739103 and BBa_I739106 which are parts of composites BBa_I739006, BBa_I739016 and BBa_I739010 respectively.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 291