Part:pSB3K1:Design

pACYC177 converted into BioBrick vector (formerly BBa_I739202)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3043
  Illegal NheI site found at 1305
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 3049
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 3043
  Illegal BamHI site found at 3037
  Illegal XhoI site found at 1669
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 3043
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 3043
  Plasmid lacks a suffix.
  Illegal XbaI site found at 3058
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 895
  Illegal AgeI site found at 1219
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 190
  

Design Notes

• RS 23:12, 3 September 2008 (UTC): The default insert for this vector is Part:BBa_I739400. The physical location for this vector needs to be remapped to Part:BBa_I739400. pSB3K1 is the vector that contains Part:BBa_I739400.

Source

Bioprocess Laboratory at ETH Zurich