Part:pSB3K1

No part name specified with partinfo tag.

Plasmid pACYC177BB1 (Fig. 1) is based on pACYC177 (Fig. 2), a low-copy cloning vector (10-12 copies per cell) that confers ampicillin (ApR) and kanamycin (KmR) resistance and harbours the p15A origin of replication.

Fig. 1: Map of the pCK01BB1 plasmid

Fig. 2: Map of the original pCK01 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. Since the original plasmid also contains a SpeI restriction site in the origin of replication, the ACT codon at this position was changed to ATT by site directed mutagenesis. The term "BB1" in pCK01BB1 refers to "BioBrick version 1".

Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 4, Part 5new, Part 8, Part 9 and the composites BBa_I739015, BBa_I739016 and BBa_I739017. More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here].

Sequence and Features No part name specified with partinfo tag.